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錦玟儀器:沒有低氧培養箱,怎么模擬低氧條件下培養細胞?

1.低氧模型:只需一個真空干燥器、一個測氧儀和一罐缺氧氣體(95 % N2∶5 % CO2),祛除真空干燥器的玻璃塞,裝上一個橡皮塞,塞上打兩個孔,一個是進氣孔,一個是出氣孔,進氣孔通缺氧氣體,出氣孔水封,以一定流量通氣一端時間后(視干燥器大小而定),用測氧儀在出氣口端測氧濃度,控制氧濃度小于1%。。

2.最精密、準確的缺氧裝置就是買一套乏氧培養箱,不過一般實驗室可達不到這個條件,比較貴;

3.氯化鈷處理 細胞模擬缺氧

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日本a级黄毛片免费天堂

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